Gunmen reportedly set up roadblocks along the highway on Sunday, targeting vehicles and individuals travelling through both Gbimsi and Walewale Town.
Travellers caught in the blockade faced intense scrutiny as the assailants checked identities to identify specific targets.
In the attack, eight people lost their lives, and two vehicles were set on fire, prompting alarm over highway security.
One survivor, recounting his ordeal, said, "I was travelling from Sunyani to Paga.